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Image Search Results
Journal: Nature Communications
Article Title: Preservation of microvascular barrier function requires CD31 receptor-induced metabolic reprogramming
doi: 10.1038/s41467-020-17329-8
Figure Lengend Snippet: WT and cd31 −/− EC monolayers were stimulated by MHC antibody-ligation or treated with an Isotype-matched control and secondary antibodies (2 h). Some cd31 −/− ECs were also treated with an Akt activator (500 nM, 3 h) prior to antibody stimulation. Vehicle was added in the untreated cultures (IsC and MHC ligation). a β-catenin and cMyc expression were determined by immunofluorescent antibody staining and wide-field microscopy. The nucleus was stained with DAPI. The mean fluorescence intensity of cMyc and β-catenin measured in 500 cells in three independent experiments is shown in ( b , c ), respectively. Scale bar = 40 μm. Data are mean ± SD. one-way Anova with Tuckey post-hoc test. b WT IsC vs WT MHC; c WT IsC vs WT MHC *** p = 0.0008, cd31 − / − IsC + C991 vs cd31 − / − MHC + C991 *** p = 0.0002. d , e : cMyc ( d ) and aldolase ( e ) gene transcription by WT (upper panels) and cd31 −/− (lower panels) EC at the indicated time points. n = 3 biologically independent samples, N = 2 independent experiments. Error bars represent SD. One-way Anova with Tuckey post-hoc test or T -test ( d , e ). d WT IsC 30′ vs WT MHC 30′ ** p = 0.003, WT IsC 120′ vs WT MHC 120′ **** p < 0.0001; e WT IsC 30′ vs WT MHC 30′ *** p = 0.0008, WT IsC 120′ vs WT MHC 120′ **** p < 0.0001, WT IsC 240′ vs WT MHC 240′ **** p < 0.0001.
Article Snippet: Coverslips were extensively washed, air dried, and mounted in Vectorshield (Vector Laboratories, H-5000) mounting medium for
Techniques: Ligation, Expressing, Staining, Microscopy, Fluorescence